Team:GIFU TOKAI/Basic Parts
Basic Parts
Basic parts' collection
BBa_K3222000
Description of this part
T7 promoters can produce rich amount of RNA from DNA using T7 RNA Polymerase. T7 promoters have unique sequence which can be only recognized by T7 RNAP. The promoter is typically used for production of protein in E.coli, especially in BL21 (DE3). This year, iGEM GIFU TOKAI team tried to generate linear mRNA in vitro and used in vitro transcription kit. As minimal sequence for T7 promoter, we need TAATACGACTCACTATAGGG. In iGEM registry of T7 promoter collection, only TAATACGACTCACTATAGG and TAATACGACTCACTATAG were found. Thus, we decided to compare efficiency of transcription of RNA of each promoter.